sfGFP熒光蛋白的激發(fā)波長(zhǎng)和發(fā)射波長(zhǎng)
sfGFP的激發(fā)波長(zhǎng)主峰在488nm，發(fā)射波長(zhǎng)主峰在507nm。
上海路陽(yáng)儀器有限公司現(xiàn)貨銷售進(jìn)口便攜式熒光蛋白激發(fā)光源，又叫熒光蛋白觀察燈、GFP激發(fā)光源，這個(gè)便攜式光源能夠激發(fā)GFP、EGFP、BFP、CFP、YFP、RFP、Dsred，mcherry等各種熒光蛋白的熒光，常備現(xiàn)貨的有綠色熒光蛋白、紅色熒光蛋白、黃色熒光蛋白激發(fā)光源咨
常用的報(bào)告基因包括：lacZ、Egfp 、zsGreen、EYFP、DsRed (DsRed2)、mRFP、mCherry、HuCD2、Thy1.1、HA tag、FLAG tag、Luciferase等。zsGreen和EGFP, EYFP都是綠色熒光。EGFP和EYFP很難區(qū)分開。zsGreen是一個(gè)比較穩(wěn)定的綠色熒光蛋白。DsRed和TdTomato都是紅色熒光。TdTomato是一個(gè)信號(hào)非常強(qiáng)的熒光蛋白，對(duì)細(xì)胞/小鼠也沒(méi)有明顯的毒性。mRFP和mCherry是兩個(gè)非常好的熒光蛋白，需要530nm的激發(fā)光，所以可以用LUYOR-3415RG熒光蛋白觀察燈激發(fā)，能夠用肉眼觀察到熒光蛋白在小鼠上的表達(dá)。
上海路陽(yáng)儀器有限公司銷售美國(guó)路陽(yáng)便攜式熒光蛋白激發(fā)光源、熒光手電筒，能夠激發(fā)各種熒光蛋白在動(dòng)植物上的轉(zhuǎn)基因表達(dá)，如果您要觀察GFP熒光蛋白在植物葉片、愈傷、種子、根系的轉(zhuǎn)基因表達(dá)。上海路陽(yáng)提供樣機(jī)免費(fèi)試用，滿意付款。我們還在北京、鄭州、海口、武漢、哈爾濱設(shè)有代理商，可以提供上門演示。詳情請(qǐng)惦記店鋪查詢
sfGFP--Superfolder GFP
Molecular engineering of the green fluorescent protein (GFP) into a robust and stable variant named Superfolder GFP (sfGFP) has revolutionized the field of biosensor development and the use of fluorescent markers in diverse area of biology. sfGFP-based self-associating bipartite split-FP systems have been widely exploited to monitor soluble expression in vitro, localization, and trafficking of proteins in cellulo. A more recent class of split-FP variants, named ? tripartite ? split-FP, that rely on the self-assembly of three GFP fragments, is particularly well suited for the detection of protein-protein interactions. In this review, we describe the different steps and evolutions that have led to the diversification of superfolder and split-FP reporter systems, and we report an update of their applications in various areas of biology, from structural biology to cell biology.
What is Superfolder GFP?
Superfolder GFP is a basic (constitutively fluorescent) green fluorescent protein published in 2005, derived from Aequorea victoria. It is reported to be a very rapidly-maturing weak dimer. +.
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